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20x dUTP UDGase Additive

: PB20.51


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Pack Size

500 reactions 

Features

  • Compatible with PCR Biosystems qPCR DNA kit
  • 20x blended dUTP and uracil DNA glycosylase
  • dUTP concentration ensures complete removal of contaminating PCR products

20x dUTP UDGase is compatible with PCR Biosystems range of real-time PCR mixes. The reagent contains dUTP, stabilizers and uracil DNA glycosylase (UDGase). The dUTP concentration ensures sufficient incorporation for complete removal of contaminating PCR products.

Carry-over contamination can be prevented by incorporating dUTP in all PCR products and treating all subsequent fully preassembled starting reactions with UDGase, followed by thermal inactivation of UDGase. UDGase cleaves the uracil base from the phosphodiester backbone of uracil-containing DNA, but has no effect on natural (i.e., thymine-containing) DNA. The resulting apyrimidinic sites block replication by DNA polymerases, and are very labile to acid/base hydrolysis. Because UDG does not react with dUTP, and is also inactivated by heat denaturation prior to the actual PCR, carry-over contamination of PCRs can be controlled effectively if the contaminants contain uracils in place of or in addition to thymines.

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