Enhanced DNA polymerases

PCR Biosytems has increased the efficiency of our DNA polymerases by increasing the positive charge at key residues involved in DNA binding. A combination of rational mutagenesis and medium throughput screening has dramatically increased incorporation efficiency of all our polymerases. For the end user this means higher PCR product yields, less PCR inhibition from template contaminants and a higher success rate for “difficult” reactions.

Reaction buffers developed for minimal user optimisation

Buffer system, pH, salts, blend of enhancers and stabilizers are all critical for efficient PCR.For the development of each PCR Biosystems kit a high throughput approach was used to screen hundreds of thousands of reagent combinations on a comprehensive set of PCR primers and templates. The resulting unique combination of reagents in our reaction buffers dramatically increases PCR success rate, yield and efficiency.

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